Search results for "Exhaled breath condensate"

Is Health-Related Quality of Life Associated with Upper and Lower Airway Inflammation in Asthmatics?

2013

Background.Allergic diseases impair health-related quality of life (HR-QoL). However, the relationship between airway inflammation and HR-QoL in patients with asthma and rhinitis has not been fully investigated. We explored whether the inflammation of upper and lower airways is associated with HR-QoL.Methods.Twenty-two mild allergic asthmatics with concomitant rhinitis (10 males, 38 ± 17 years) were recruited. The Rhinasthma was used to identify HR-QoL, and the Asthma Control Test (ACT) was used to assess asthma control. Subjects underwent lung function and exhaled nitric oxide (eNO) test, collection of exhaled breath condensate (EBC), and nasal wash.Results.The Rhinasthma Global Summary sc…

Allergen-specific Immunotherapy with Purified nAlt a1: Effects on AMP Responsiveness, Exhaled Nitric Oxide and Exhaled Breath Condensate pH

2010

Multipumping Nitrite Determination in Exhaled Breath Condensate

2006

Abstract A flow system based on multicommutation is proposed for the rapid, clean, and inexpensive determination of nitrites in small volumes of breath condensates. The procedure exploits the colorimetric detection of nitrite with the Griess reagent [0.03% naphthylethylene diamine dihydrochloride (NED), 0.5% sulpfhanilamide, and 3.0% phosphoric acid] in acidic medium at 540 nm correcting the variations of the baseline with measurements at 424 nm. The flow system was designed with a set of solenoid micropumps to minimize sample and reagent consumption and waste generation. The detection limit was estimated as 3.8 ng mL−1 (99.7% confidence level) with a linear response ranging up to 500 ng mL…

Noninvasive methods for the detection of upper and lower airway inflammation in atopic children

2006

Background Exhaled nitric oxide (FE NO ) and exhaled breath condensate (EBC) are noninvasive methods to assess inflammation. Objective To investigate the role of the FE NO and of the EBC pH and IL-5 levels in atopic children. Methods We evaluated oral and nasal FE NO and the pH and IL-5 of oral and nasal EBC in children with atopic dermatitis (AD; n=18), allergic rhinitis (AR; n=18), intermittent asthma (n = 21), moderate persistent asthma (n = 18), and healthy controls (HCs; n=16). Results Oral FE NO was significantly increased in asthma, whereas the nasal values were increased in AR and asthma in comparison with HCs. The pH of oral EBC was lower in AD and asthma than in AR and HCs, wherea…

Effect of nebulized beclomethasone on airway inflammation and clinical status of children with allergic asthma and rhinitis: a randomized, double-bli…

2012

We aimed to evaluate the therapeutic effect of nebulized beclomethasone dipropionate (nBDP) on both allergic asthma and rhinitis. In a randomized, double-blind, placebo-controlled study, 40 children (mean age 10.7 ± 2.1 years) with allergic asthma and rhinitis received either nBDP (daily dose of 800 µg, administered twice daily) or placebo for 4 weeks (with a face mask), after a 2-week run-in period of clinical assessment. Nasal and oral fractional exhaled nitric oxide (FeNO) measurements together with pulmonary function tests, nasal and oral exhaled breath condensate (EBC) collection for pH and interleukin-5 (IL-5) measurements as well as nasal and bronchial symptom scores were obtained at…

Early detection of lung cancer in exhaled breath condensate using miRNA markers

2015

Background: Lung cancer is the leading cause of death by cancer worldwide. Since 5-year survival rate increases significantly when lung cancer is diagnosed at early stages, the development of accurate non-invasive biomarkers for early lung cancer diagnosis is of utmost importance. Over the last years, several tumour biomarkers based on microRNAs (miRNAs) determined in exhaled breath condensate (EBC) have been evaluated and could be applied to early diagnosis of lung cancer. Rationale: miRNA signatures for surgical specimens of lung cancer have been determined providing a panel of differentially expressed miRNAs that were able to discriminate lung cancer patients from normal subjects. Using …

Environment Exposure to Pollutants

2015

Effects of exposure to welding fume on lung function: results from the German WELDOX study. M. Lehnert, F. Hoffmeyer, K. Gawrych, A. Lotz, E. Heinze, H. Berresheim, R. Merget, V. Harth, R. Van Gelder, J.-U. Hahn, A. Hartwig, T. Weiss, B. Pesch, and T. Bruning, for the WELDOX Study Group Disability-Adjusted Life Years in the Assessment of Health Effects of Traffic-Related Air Pollution. L. Adamkiewicz, A.J. Badyda, A. Gayer, and D. Mucha Impact of Traffic-Related Air Pollution on Health. J. Jakubiak-Lasocka, J. Lasocki, R. Siekmeier, and Z. Chlopek Traffic-related air pollution and respiratory tract efficiency. A.J. Badyda, P. Dabrowiecki, P.O. Czechowski, G. Majewski, and A. Doboszynska Dif…

Oxidative lung injury correlates with one-lung ventilation time during pulmonary lobectomy: a study of exhaled breath condensate and blood.

2015

OBJECTIVES: During lung lobectomy, the operated lung is collapsed and hypoperfused; oxygen deprivation is accompanied by reactive hypoxic pulmonary vasoconstriction. After lung lobectomy, ischaemia present in the collapsed state is followed by expansion–reperfusion and lung injury attributed to the production of reactive oxygen species. The primary objective of this study was to investigate the time course of several markers of oxidative stress simultaneously in exhaled breath condensate and blood and to determine the relationship between oxidative stress and one-lung ventilation time in patients undergoing lung lobectomy. METHODS: This single-centre, observational, prospective study includ…

Effects of cigarette smoke on methacholine- and AMP-induced air trapping in asthmatics.

2014

Abstract Objective: No information is available on the effect of cigarette smoke on bronchoconstrictor-induced air trapping in asthma. The aim of this study was to evaluate the additional influence of smoking on methacholine- and adenosine 5'-monophosphate (AMP)-induced air trapping in subjects with asthma.Airway responsiveness to methacholine and AMP, bronchial (J'awNO) and alveolar (CANO) nitric oxide (NO) and exhaled breath condensate pH were measured in 68 adults (23 current smokers with asthma, 23 non-smokers with asthma and 22 current or former smokers with chronic obstructive pulmonary disease; COPD). The degree of air trapping induced by each bronchoconstrictor agent was expressed b…

The effect of different periods of argon deaeration on exhaled breath condensate pH.

2011

Exhaled breath condensate (EBC) pH has been considered as a biomarker of airway inflammation in asthma. However, little information is available on the duration of argon deaeration required to achieve a stable pH in EBC samples.To identify differences in EBC pH after argon deaeration for 2, 4, and 8 min.EBC pH was determined in EBC samples from 48 subjects with allergic rhinitis (11 asthmatics) and 14 healthy volunteers without deaeration and after argon deaeration for 2, 4, and 8 min.The mean (95% CI) pH values obtained from samples analyzed after 4 min [7.66 (7.52-7.80)] and 8 min [7.70 (7.55-7.85)] of argon deaeration were significantly less acidic (p.001) than those identified after 2 m…